UNIVERSITY OF NEBRASKA MEDICAL CENTER- Omaha, Nebraska

A NIH-funded postdoctoral position is available in the laboratory of Dr. Tammy Kielian in the Department of Pathology and Microbiology, University of Nebraska Medical Center (UNMC) in Omaha, Nebraska (https://www.unmc.edu/pathology/faculty/bios/kielian.html). The candidate will direct independent projects related to the role of innate and adaptive immunity in host defense against Staphylococcus aureus using a mouse model of S. aureus craniotomy infection as well as translational research with clinical specimens from patients with craniotomy infections. Our laboratory utilizes numerous tools to explore host-pathogen crosstalk, including single cell RNA-seq, epigenetic platforms, and transposon sequencing. Twitter: @KielianLab

UNMC has a vibrant research and medical community within the growing and affordable city of Omaha, Nebraska. The Department of Pathology and Microbiology has over 60 faculty members, nearly 100 graduate students, postdoctoral fellows, and residents working in modern, well-equipped labs with state-of-the-art facilities. The Staphylococcal Research Group at UNMC (https://www.unmc.edu/pathology/csr/) offers a collaborative multi-disciplinary environment for the study of staphylococcal infections.

The successful candidate should possess a PhD in neuroimmunology or a related background and have excellent written and oral communication skills. Salary will be commensurate with experience.

Interested persons should send a Curriculum vitae, summary of research experience and interests, and contact information of three references to Tammy Kielian, PhD, via an online application through the University of Nebraska Medical Center employment website at https://unmc.peopleadmin.com/postings/66883. Applications will be reviewed as they are received and will continue until the position is filled.

Position title: Assistant Professor-Fixed Term – Imaging Specialist

Organization: Michigan State University

Location: East Lansing, MI, USA

Summary of position
The Gulbransen and Jackson labs use advanced cellular imaging and genetic approaches to study interactions between neurons and non-neuronal cells in the periphery. Major techniques used in these studies include network- and cellular-level calcium imaging with GCaMPs, selective cell stimulation using chemogenetics, cell labeling assays, fluorescent reporter systems, RNA sequencing, and physiological assays of function.

The successful candidates will be expected to conduct experiments aimed at understanding the neurohumoral regulation of perivascular adipose tissue, collect and analyze data from these experiments, and report the results of these studies at scientific meetings and in publications (70% effort). The successful candidate will also be expected to oversee the daily operations of a microscopy core facility which houses a Zeiss LSM 880 NL microscope with Airyscan detectors, train users in the use of this microscope; train and assist with image analysis; and help facilitate collaborative research effort using this system (30% effort). There will also be opportunities to participate in professional development programs and to write and submit grants and career development awards.

Qualified candidates must have a Ph.D. in Neuroscience, Physiology, Pharmacology, Physics, Immunology, or a related field. Candidates must also have at minimum of 2 years of postdoctoral training in live cell imaging using confocal, 2- or multiphoton imaging, or similar methods. Candidates must have demonstrated experience in image analysis and processing and should have a track record of research in, or complementary to vascular physiology or pharmacology, neurobiology, immunology or related fields. The candidate must have excellent oral and written communication skills, and to be willing to work with a diverse group of investigators.

This position is a 12-month fixed term appointment with opportunity for renewal based on performance and funding.

Interested candidates should submit the following application materials: 1) a letter of intent outlining their qualifications and career objectives, 2) curriculum vitae, 3) three letters of reference. Inquiries should be directed to Dr. Brian Gulbransen at gulbrans@msu.edu or to Dr. William Jackson at jacks783@msu.edu. Formal applications should be submitted through the MSU applicant portal:

Review of applications will begin immediately and continue until the position is filled.

Link to full description: https://careers.msu.edu/en-us/job/510024/ast-proffixed-term-imaging-specialist

Contact information: Inquiries should be directed to Dr. Brian Gulbransen at gulbrans@msu.edu or to Dr. William Jackson at jacks783@msu.edu

Postdoctoral positions are available for 2-3 years in the laboratory of Dr. Munjal Acharya funded by NIH, California Institute of Regenerative Medicine (CIRM) and the American Cancer Society (ACS). Salary will be commensurate with experience.

The scholar will be responsible for investigating regenerative strategies to remediate radiation- or chemo-therapy-related brain injury (chemobrain) and cognitive dysfunction. Additional projects will involve characterizing the impact of gliosis (microglial and astrocytic activation), neuroinflammation and CNS complement cascade activation on brain function. Our laboratory employs gene silencing, transgenic, stem cell-based and pharmacological approaches to study neurobiological mechanisms and regenerative strategies to ameliorate neuroinflammation and cognitive impairments.

Requirements:

Successful candidates will have an excellent track record and be highly motivated scientist who can carry out independent experiments and work well with a team. The successful candidate will play a critical role in contributing to execution of the overall aims of the project and the analysis and preparation of the data for presentation in scientific meetings and pee-reviewed publications.

Required skills:

• A Ph.D and laboratory experience in neuroscience, molecular biology and/or biochemistry.
• Hands on experience with mouse or rat handling, rodent behavior and cognitive function.
• Standard molecular and cell biology techniques (IHC, ELISA, Western blot, RNA, DNA).
• Strong skill in organization, data handling and statistical analysis.
• Supervise/train students and research staff.
• Proficiency in English with excellent oral, written and interpersonal communication skills.

Desirable skills:

• Brain cancer induction (orthotopic and flank cancer models), cancer imaging (BLI, CBCT)
• Hands on experience with mouse and/or rat stereotaxic surgery
• Mammalian cell culturing (human or rodent stem cells, cancer cells), isolation and characterization of extracellular vesicles (exosomes)
• Confocal and/or Super-Resolution microscopy, and 3D fluorescent image quantification techniques (i.e. Imaris, Auto Quant, ClearView, Neurolucida, Stereology).
• Knowledge of genetic manipulation of mammalian cells and mouse brain to knockdown or overexpress genes (AAV, Lentivirus, CRISPR-Cas, Cre-LoxP) Electrophysiology experience would be advantageous

Send CV with the names and addresses of three references (please do not solicit letters) to:Dr. Munjal Acharya macharya@uci.edu

To apply go to https://recruit.ap.uci.edu/JPF07131